Demonstration of a COOH-terminal extension on equine lutropin by means of a common acid-labile bond in equine lutropin and equine chorionic gonadotropin.
نویسندگان
چکیده
The beta subunits of equine lutropin and equine chorionic gonadotropin were incubated in 0.013 N HCl for 30 min at 110 degrees C and separated into two fragments by reverse-phase high performance liquid chromatography. The amino acid and carbohydrate compositions of both fragments from each subunit were analyzed. The results demonstrated that equine lutropin-beta has a glycosylated COOH-terminal extension that differs only in carbohydrate composition from the COOH-terminal portion of equine chorionic gonadotropin-beta. This is the first demonstration of a glycosylated COOH-terminal extension in a pituitary glycoprotein hormone.
منابع مشابه
Pregnant mare serum gonadotropin. An in vitro biological characterization of the lutropin-follitropin dual activity.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 260 17 شماره
صفحات -
تاریخ انتشار 1985